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Colorimetric Method for the Determination of Proteins Using Immobilized Microbial Protease and a Smartphone Camera

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00556181" target="_blank" >RIV/60162694:G44__/20:00556181 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.tandfonline.com/doi/abs/10.1080/00032719.2020.1792477" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/00032719.2020.1792477</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1080/00032719.2020.1792477" target="_blank" >10.1080/00032719.2020.1792477</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Colorimetric Method for the Determination of Proteins Using Immobilized Microbial Protease and a Smartphone Camera

  • Original language description

    The determination of proteins is very important in industry, medicine, analytical chemistry, and biotechnology. A novel colorimetric method using magnetic microparticles as a carrier of immobilized microbial protease on surface was developed and smartphone camera was employed for detection. Folin and Ciocalteu's phenol reagent was selected as the chromogen and casein from bovine milk was used as the substrate. Casein was cleaved by immobilized enzyme to tyrosine, which together with chromogen spontaneously generated a blue color measurable by a spectrophotometer or photographic detection by a smartphone camera. The photographs were processed by a computer and selected points were transformed into RGB numeric values. A reference spectrophotometric method provided results that provide a Michaelis-Menten dependence with the Michaelis-Menten constant equal to 0.11 mmol/l and a limit of detection equal to 41 mu g/ml. The novel method using spectrophotometric detection shows the same Michaelis-Menten dependence with a Michaelis-Menten constant equal to 0.11 mmol/l with a limit of detection of 4.27 mu g/ml. The RGB method using a smartphone for the determination of proteins was demonstrated to be an alternative to the standard spectrophotometer. The limit of detection was 242 mu g/ml, which is sufficient for protein determination in real samples. Easy processing, good analytical results, and low demands on equipment make this method a suitable tool for a wide range of applications in industry, analytical chemistry, medicine, biotechnology, and biosensors.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Letters

  • ISSN

    0003-2719

  • e-ISSN

    1532-236X

  • Volume of the periodical

    54

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    1023-1037

  • UT code for WoS article

    000547713100001

  • EID of the result in the Scopus database

    2-s2.0-85087859715