Colorimetric Method for the Determination of Proteins Using Immobilized Microbial Protease and a Smartphone Camera

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00556181" target="_blank" >RIV/60162694:G44__/20:00556181 - isvavai.cz</a>

Result on the web

<a href="https://www.tandfonline.com/doi/abs/10.1080/00032719.2020.1792477" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/00032719.2020.1792477</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00032719.2020.1792477" target="_blank" >10.1080/00032719.2020.1792477</a>

Result language

angličtina

Original language name

Colorimetric Method for the Determination of Proteins Using Immobilized Microbial Protease and a Smartphone Camera

Original language description

The determination of proteins is very important in industry, medicine, analytical chemistry, and biotechnology. A novel colorimetric method using magnetic microparticles as a carrier of immobilized microbial protease on surface was developed and smartphone camera was employed for detection. Folin and Ciocalteu's phenol reagent was selected as the chromogen and casein from bovine milk was used as the substrate. Casein was cleaved by immobilized enzyme to tyrosine, which together with chromogen spontaneously generated a blue color measurable by a spectrophotometer or photographic detection by a smartphone camera. The photographs were processed by a computer and selected points were transformed into RGB numeric values. A reference spectrophotometric method provided results that provide a Michaelis-Menten dependence with the Michaelis-Menten constant equal to 0.11 mmol/l and a limit of detection equal to 41 mu g/ml. The novel method using spectrophotometric detection shows the same Michaelis-Menten dependence with a Michaelis-Menten constant equal to 0.11 mmol/l with a limit of detection of 4.27 mu g/ml. The RGB method using a smartphone for the determination of proteins was demonstrated to be an alternative to the standard spectrophotometer. The limit of detection was 242 mu g/ml, which is sufficient for protein determination in real samples. Easy processing, good analytical results, and low demands on equipment make this method a suitable tool for a wide range of applications in industry, analytical chemistry, medicine, biotechnology, and biosensors.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Letters

ISSN

0003-2719

e-ISSN

1532-236X

Volume of the periodical

54

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

1023-1037

UT code for WoS article

000547713100001

EID of the result in the Scopus database

2-s2.0-85087859715