The Determination of Lipase Activity by Measuring pH Using ion-Sensitive Field-effect Transistor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F21%3A00557110" target="_blank" >RIV/60162694:G44__/21:00557110 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/21:10442621

Result on the web

<a href="http://www.electrochemsci.org/papers/vol16/210760.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol16/210760.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2021.07.59" target="_blank" >10.20964/2021.07.59</a>

Result language

angličtina

Original language name

The Determination of Lipase Activity by Measuring pH Using ion-Sensitive Field-effect Transistor

Original language description

Lipases play an important role in the human metabolism and their abnormal function appear to be associated with many pathological conditions including obesity, Alzheimer disease, atherosclerosis, dyslipideamia associated with diabetes, some infections etc. Measurement of lipase activity therefore becomes important for instrumental diagnosis of the mentioned pathologies. In this work, lipase activity was measured using ion-sensitive field-effect transistor (ISFET) enzyme biosensor based on Tween 20 as a substrate. Developed biosensor is based on measurement of pH change induced by reaction of lipase with Tween 20 when potentiometric ISFET was used for determination of pH changed by the analyte. Tween 20 in concentration range from 0 to 4 % was measured for determination of optimal Tween 20 concentration. Limit of detection was set to be 0.02 %and result curve showed Michaelis-Menten dependency with the Michaelis-Menten constant equal to 0.11 %. Concentration curve of lipase in concentration range from 0 - 4 mg/ml was measured with 2 % Tween 20. Limit of detection was calculated to be 0.02 mg/ml with correlation coefficient 0.998 and Michaelis-Menten constant was equal to 0.36 mg/ml. Effect of interferences and matrix was tested with different concentration. Novel ionsensitive field-effect transistor method is undemanding for laboratory equipment, chemicals and easy repeatable and shows good analytical results comparable with other analytical methods.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

7

Country of publishing house

RS - THE REPUBLIC OF SERBIA

Number of pages

11

Pages from-to

210760

UT code for WoS article

000669352100058

EID of the result in the Scopus database

2-s2.0-85108632050