Indoxyl Acetate as a Substrate for Analysis of Lipase Activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00555343" target="_blank" >RIV/60162694:G44__/19:00555343 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/19:10410227
Result on the web
<a href="http://downloads.hindawi.com/journals/ijac/2019/8538340.pdf" target="_blank" >http://downloads.hindawi.com/journals/ijac/2019/8538340.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2019/8538340" target="_blank" >10.1155/2019/8538340</a>
Alternative languages
Result language
angličtina
Original language name
Indoxyl Acetate as a Substrate for Analysis of Lipase Activity
Original language description
Lipases play a crucial role in metabolism of microbes, fungi, plants, and animals, and in analytical chemistry, they are often used in detection of fats and triglycerides. Determination of lipase activity is also important in toxicology, when lipase activity can be both increased and decreased by organophosphates and other pesticides and in medicine for diagnosis of heart diseases. The standard method for lipase activity determination is based on cleaving ester bonds in lipase buffer containing Tween. Our aim was to find a method with faster and more sensitive response. It is known that acetylcholinesterase belongs to the same group of hydrolases enzymes as lipases and it cleaves indoxyl acetate, so we assume indoxyl acetate could report a similar reaction with lipase. Our method is based on indoxyl acetate as a substrate for lipase, where indoxyl acetate is cleaved by lipase to indoxyl and acetate moiety and blue indigo is created. The method was optimized for different times and amount of enzyme and compared with the standard Tween assay. The calibration curve measured in reaction time 20 minutes with 10 mu l of lipase exhibited the best analytical parameters, and it showed Michaelis-Menten response with the Michaelis-Menten constant equal to 8.72 mmol/l. The indoxyl acetate-based method showed faster and more sensitive response than the standard method for lipase activity determination, so it has great potential in biosensor construction and it could be used in industry, medicine, toxicology, and common practice where the activity of lipases is need to be measured.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Analytical Chemistry
ISSN
1687-8760
e-ISSN
1687-8779
Volume of the periodical
2019
Issue of the periodical within the volume
Dec
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
8538340
UT code for WoS article
000503428200001
EID of the result in the Scopus database
2-s2.0-85076925556