Second harmonic generation imaging of the deep shade plant Selaginella erythropus using multifunctional two-photon laser scanning microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43884026" target="_blank" >RIV/60076658:12520/12:43884026 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/j.1365-2818.2012.03668.x" target="_blank" >http://dx.doi.org/10.1111/j.1365-2818.2012.03668.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1365-2818.2012.03668.x" target="_blank" >10.1111/j.1365-2818.2012.03668.x</a>

Result language

angličtina

Original language name

Second harmonic generation imaging of the deep shade plant Selaginella erythropus using multifunctional two-photon laser scanning microscopy

Original language description

Background: Multifunctional two-photon laser scanning microscopy provides attractive advantages over conventional two-photon laser scanning microscopy. For the first time, simultaneous measurement of the second harmonic generation (SHG) signals in the forward and backward directions and two photon excitation fluorescence were achieved from the deep shade plant Selaginella erythropus. Results: These measurements show that the S. erythropus leaves produce high SHG signals in both directions and the SHG signals strongly depend on the laser's status of polarization and the orientation of the dipole moment in the molecules that interact with the laser light. The novelty of this work is (1) uncovering the unusual structure of S. erythropus leaves, includingdiverse chloroplasts, various cell types and micromophology, which are consistent with observations from general electron microscopy; and (2) using the multifunctional two-photon laser scanning microscopy by combining three platforms of l

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BH - Optics, masers and lasers

OECD FORD branch

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Project

<a href="/en/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microscopy-Oxford

ISSN

0022-2720

e-ISSN

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Volume of the periodical

248

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

234-244

UT code for WoS article

000310977400004

EID of the result in the Scopus database

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