Second harmonic generation from thick leaves using the two-photon laser scanning microscope

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F09%3A00010049" target="_blank" >RIV/60076658:12640/09:00010049 - isvavai.cz</a>

Alternative codes found

RIV/67179843:_____/09:00342935

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Second harmonic generation from thick leaves using the two-photon laser scanning microscope

Original language description

A laser-scanning microscope using Second harmonic generation (SHG) as a probe is shown to produce high-resolution images of duckweed Lemna minuta leaves. These leaves are multi-cell layer thick. Second harmonic light is generated by a tightly focused short pulse laser beam and is collected by two objectives yielding forward and backward second harmonic digital images. This measurement shows that the signal of the second harmonic imaging in the forward and backward directions depends on the thickness ofthe chloroplast and that the forward-SH image was brighter than the backward-SH image. The image intensity also depended on the orientation of the chloroplast in relation to the illuminating polarization direction. Their light-induced re-orientation which was affected by the intensity of the illumination could be observed during the experiments.The novelty of this work is to establish new compact technique in which one call use the SH imaging to investigate the true architecture of the s

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Micron

ISSN

0968-4328

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

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UT code for WoS article

000265777000008

EID of the result in the Scopus database

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