Enhancing the resolution of the forward second harmonic imaging using the two-photon laser scanning microscope

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F09%3A00010029" target="_blank" >RIV/60076658:12640/09:00010029 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Enhancing the resolution of the forward second harmonic imaging using the two-photon laser scanning microscope

Original language description

The effect of increasing the numerical aperture and changing the immersion imaging medium of the objective in the forward second harmonic imaging of the grana and intergranal parts of chloroplasts in the leaves of shade moss Plagiomnium affine plant weredemonstrated. Also the enhancement of the forward second harmonic generation images and simultaneously records the forward and backward second harmonic signals were demonstrated. The second harmonic signals in the forward and backward directions are generated by a tightly focused linearly polarized femtosecond pulses laser. Conclusions: Forward second harmonic signals were successfully enhanced by capturing higher order of diffraction rays from the samples using high numerical aperture water immersionobjective.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Micron

ISSN

0968-4328

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

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UT code for WoS article

000268422400010

EID of the result in the Scopus database

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