In vitro and In vivo Association of Porcine Hepatic Cytochrome P450 3A and 2C Activities with Testicular Steroids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43884082" target="_blank" >RIV/60076658:12520/12:43884082 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1439-0531.2012.01986.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0531.2012.01986.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1439-0531.2012.01986.x" target="_blank" >10.1111/j.1439-0531.2012.01986.x</a>
Alternative languages
Result language
angličtina
Original language name
In vitro and In vivo Association of Porcine Hepatic Cytochrome P450 3A and 2C Activities with Testicular Steroids
Original language description
The aim of this study was to screen the inhibitory potential of several testicular steroids on cytochrome P450 3A (CYP3A) and 2C (CYP2C) activities in porcine liver microsomes. The microsomes used in this study were obtained from pubertal male pigs of two breeds, Landrace and Duroc. For the in vitro inhibition study, porcine microsomes were incubated in the presence of 17 beta-estradiol, 17 alpha-estradiol, androstenone, dehydroepiandrosterone and dihydrotestosterone. Both reversible and mechanism-basedinhibitions were examined. 7-benzyloxyresorufin (BR) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC) were used as substrates for CYP3A, and diclofenac and tolbutamide (TB) as substrates for CYP2C. 7-benzyloxyresorufin O-dealkylase (BROD) activity was inhibited by all tested steroids in the microsomes from Landrace pigs via mechanism-based mode, but in the microsomes from Duroc pigs, BROD activities were inhibited only in the presence of 17 beta-oestradiol. Mechanism-based inhibition of
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction in Domestic Animals
ISSN
0936-6768
e-ISSN
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Volume of the periodical
47
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
891-898
UT code for WoS article
000310790800008
EID of the result in the Scopus database
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