Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F12%3A43871569" target="_blank" >RIV/62157124:16270/12:43871569 - isvavai.cz</a>
Result on the web
<a href="http://www.intertox.sav.sk/ITX_pdf/05_03_2012/10102-Volume5_Issue_3-06_paper.pdf" target="_blank" >http://www.intertox.sav.sk/ITX_pdf/05_03_2012/10102-Volume5_Issue_3-06_paper.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/v10102-012-0024-3" target="_blank" >10.2478/v10102-012-0024-3</a>

Result language
angličtina
Original language name
Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes
Original language description
The aim of this study was to develop a robust method for the simultaneous determination of the activities of three porcine CYP450 enzymes in hepatic microsomes. A cocktail consisting of three selective CYP450 probe substrates, 7-ethoxyresorufin (CYP1A),coumarin (CYP2A) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC; CYP3A), was incubated with porcine liver microsomes. The presence of 7-ethoxyresorufin appears to significantly influence the kinetics of coumarin hydroxylation and BFC O-debenzylation. These results indicate that the use of 7-ethoxyresorufin in substrate cocktails together with coumarin and BFC should be avoided.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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