Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886830" target="_blank" >RIV/60076658:12520/14:43886830 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/jai.12429/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jai.12429/abstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jai.12429" target="_blank" >10.1111/jai.12429</a>
Alternative languages
Result language
angličtina
Original language name
Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca)
Original language description
Embryonic and larval development of the tench, Tinca tinca, is described, along with the origin and migration routes of germ cell lineage. Primordial germ cells (PGCs) potentially transmit genetic information to the next generation, and represent a powerful tool for creating a germ-line chimera within fish species. PGCs were identified by injecting synthesized mRNA, combining green fluorescent protein and the zebrafish nos1 3UTR, under the blastodisc of embryos at the 1-4 cell stage. Developmental stages were divided into five periods defined by morphological features: cleavage (40min-5h post-fertilization (hpf), blastula (3.6-12hpf), gastrula (12-20hpf), segmentation (20-65hpf), and hatching (65-188hpf). The migration pathways of fluorescent PGCs weredetected from 100% epiboly (18hpf) to the end of the hatching period (184hpf) in 69.3% of injected embryos, which migrated to the site of future gonads. Each hatching larva possessed 3-10 labeled PGCs with 95.1% of these cells localized
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Ichthyology
ISSN
0175-8659
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
Supplement 1
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
29-39
UT code for WoS article
000337527600007
EID of the result in the Scopus database
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