Migration of Primordial Germ Cells During Late Embryogenesis of Pikeperch Sander lucioperca Relative to Blastomere Transplantation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43892141" target="_blank" >RIV/60076658:12520/17:43892141 - isvavai.cz</a>

Result on the web

<a href="http://www.agriculturejournals.cz/web/cjas.htm?volume=62&firstPage=121&type=publishedArticle" target="_blank" >http://www.agriculturejournals.cz/web/cjas.htm?volume=62&firstPage=121&type=publishedArticle</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/40/2016-CJAS" target="_blank" >10.17221/40/2016-CJAS</a>

Result language

angličtina

Original language name

Migration of Primordial Germ Cells During Late Embryogenesis of Pikeperch Sander lucioperca Relative to Blastomere Transplantation

Original language description

Pikeperch Sander lucioperca is a valuable fish in Europe, and basic information about its embryonic development, especially primordial germ cell (PGC) migration, is important for use in biotechnology. We categorized pikeperch embryonic development into six stages as in other fish species: zygote, cleavage, blastula, gastrula, segmentation, and hatching and described PGC migration. PGCs were visualized by injection of synthesized green fluorescent protein (GFP) within the 3&apos; untranslated region (UTR) mRNA of nanos3. GFP-positive PGCs appeared in all embryos at approximately 100% epiboly. Time-lapse imaging revealed the PGC migration pattern from their initial appearance to location at the gonadal ridge. We conducted blastomere transplantation (BT) at the blastula stage. Donor embryos were labelled with GFP-nos3 3&apos; UTR mRNA and tetramethylrhodamine dextran to label PGCs and somatic cells, respectively. Twelve BT chimeras were produced, with eight surviving to hatching. All exhibited donor-derived somatic cells in the developing body. The PGCs from donor embryos were observed to migrate towards the gonad region of the host embryos. Our results indicated that BT can be successfully applied in pikeperch, and these findings may be useful to produce germline chimeras in percids.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science : Živočišná výroba

ISSN

1212-1819

e-ISSN

—

Volume of the periodical

62

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

121-129

UT code for WoS article

000396022000004

EID of the result in the Scopus database

2-s2.0-85016310948