Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890495" target="_blank" >RIV/60076658:12520/16:43890495 - isvavai.cz</a>
Result on the web
<a href="http://www.agriculturejournals.cz/publicFiles/187820.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/187820.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/35/2015-CJAS" target="_blank" >10.17221/35/2015-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation
Original language description
Recently, transplantation of germ cells has attracted attention as a potential technique for efficient reproduction of fish. One of the well-proven techniques to deliver donor germ cells into a recipient is the transplantation of primordial germ cells (PGCs) during the blastula stage. Nevertheless, the application of such techniques so far has been limited to model fish species such as zebrafish, due to the lack of information about early development in many fish species. We propose that pikeperch (Sander lucioperca) can be a useful model species for establishing this technique in the order Perciformes, which includes commercially and ecologically important marine species. In this study, we described the important events, namely, embryonic staging, yolk syncytial layer (YSL) formation, and midblastula transition (MBT) during the blastula stage in pikeperch to obtain basic information about early embryonic development. The chorion was softened by treating with 0.2% trypsin and 0.4% urea in Ringer's solution so as to remove it easily by forceps. Although the first cleavage occurred at about 2.5 h post fertilization, blastomeres divided approximately every one hour after this at 15 degrees C. The YSL was formed after the breakdown of marginal cells during the 512- to 1k-cell stage. Cell division analysis by 4'-6-diaminido-2-phenylindole (DAPI) staining revealed that transition from synchronous to asynchronous division occurred after the 10th cleavage (1k-cell stage). Our results indicate that zygotic gene expression (MBT) starts after this stage. Next, we performed blastodisc isolation assay to find the competent stage for embryonic manipulation. Embryos were manipulated by using a microneedle every hour from the 512-cell to the sphere stage, and then developmental rates were evaluated at the hatching stage. The highest survival rate was obtained when we performed this manipulation at the 1k-cell stage.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science : Živočišná výroba
ISSN
1212-1819
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
6
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
273-280
UT code for WoS article
000382555000003
EID of the result in the Scopus database
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