Microscope point spread function, focus and calculation of optimal microscope set-up
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886876" target="_blank" >RIV/60076658:12520/14:43886876 - isvavai.cz</a>
Result on the web
<a href="http://www.tandfonline.com/doi/abs/10.1080/00207160.2013.851379#.VKqbbntlw80" target="_blank" >http://www.tandfonline.com/doi/abs/10.1080/00207160.2013.851379#.VKqbbntlw80</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00207160.2013.851379" target="_blank" >10.1080/00207160.2013.851379</a>
Alternative languages
Result language
angličtina
Original language name
Microscope point spread function, focus and calculation of optimal microscope set-up
Original language description
If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. Wewant to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF COMPUTER MATHEMATICS
ISSN
0020-7160
e-ISSN
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Volume of the periodical
91
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
221-232
UT code for WoS article
000335089900006
EID of the result in the Scopus database
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