Transcripts of genes encoding reproductive neuroendocrine hormones and androgen receptor in the brain and testis of goldfish exposed to vinclozolin, flutamide, testosterone, and their combinations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890481" target="_blank" >RIV/60076658:12520/16:43890481 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007/s10695-016-0205-7" target="_blank" >http://link.springer.com/article/10.1007/s10695-016-0205-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-016-0205-7" target="_blank" >10.1007/s10695-016-0205-7</a>

Result language

angličtina

Original language name

Transcripts of genes encoding reproductive neuroendocrine hormones and androgen receptor in the brain and testis of goldfish exposed to vinclozolin, flutamide, testosterone, and their combinations

Original language description

Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 mu g/L), anti-androgen flutamide (Flu, 300 mu g/L), and androgen testosterone (T, 1 mu g/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gn-rh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 mu g/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 mu g/L VZ with Flu, 100 mu g/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 mu g/L VZ with Flu and 100 mu g/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 mu g/L VZ, to Flu, and to combinations of 30 mu g/L VZ with Flu, 100 mu g/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 mu g/L VZ with Flu, 100 mu g/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 mu g/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

1157-1165

UT code for WoS article

000379734500008

EID of the result in the Scopus database

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