Lipid composition in common carp (Cyprinus carpio) sperm possessing different cryoresistance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890532" target="_blank" >RIV/60076658:12520/16:43890532 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.cryobiol.2016.08.005" target="_blank" >http://dx.doi.org/10.1016/j.cryobiol.2016.08.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2016.08.005" target="_blank" >10.1016/j.cryobiol.2016.08.005</a>

Result language

angličtina

Original language name

Lipid composition in common carp (Cyprinus carpio) sperm possessing different cryoresistance

Original language description

The present study examined the lipid composition of plasma membranes in carp sperm with different post-thaw motility. The approach adapted for carp sperm cryopreservation, which involves the selection of the most effective protocol for individual males by comparing two cryoprotective media, was applied to the cryopreservation procedure. Sperm motility prior to freezing was greater than 80% but decreased to 40% in one group and to 10% in another group following cryopreservation. Lipid content of fresh sperm in all groups was analysed by thin layer chromatography and gas chromatography, with significant differences in phospholipid content, cholesterol and free fatty acids detected between groups, whereas the cholesterol/phospholipid ratio was extremely similar between groups (0.52 +/- 0.038 and 0.52 +/- 0.022). Increasing concentrations of saturated fatty acids, monounsaturated acids and decreasing concentrations of polyunsaturated n-6 fatty acids were negatively correlated (P < 0.05) with post-thaw motility of the carp sperm.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cryobiology

ISSN

0011-2240

e-ISSN

—

Volume of the periodical

73

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

282-285

UT code for WoS article

000384388600026

EID of the result in the Scopus database

—