Metabolic stress leads to divergent changes in the ghrelinergic system in goldfish (Carassius auratus) gonads

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899272" target="_blank" >RIV/60076658:12520/19:43899272 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S1095643318303635" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1095643318303635</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpa.2019.05.027" target="_blank" >10.1016/j.cbpa.2019.05.027</a>

Result language

angličtina

Original language name

Metabolic stress leads to divergent changes in the ghrelinergic system in goldfish (Carassius auratus) gonads

Original language description

Various endocrine factors that regulate energy homeostasis are also implicated in the reproductive physiology of mammals. However, the hormonal link between metabolism and reproduction in fish is poorly understood. Ghrelin is a multifunctional hormone with both metabolic and reproductive roles in vertebrates. Post-translational acylation by ghrelin-O-acyltransferase (GOAT) is critical for its biological actions. The expression of ghrelin, ghrelin or growth hormone secretagogue receptor (GHSR), and GOAT (which forms the ghrelinergic system) in fish under metabolic stress remains unclear. In this research, we used RT-qPCR and Western blot analysis to determine the expression of the ghrelinergic system in goldfish (during the reproductively active phase) hypothalamus and gonads under 7 and 28 days of fasting. We found a significant increase in preproghrelin mRNA expression in the ovary, and GOAT mRNA expression in the testis of goldfish deprived of food for 7 days. In fish deprived of food for 28 days, preproghrelin, GHSR and GOAT mRNA expression was significantly increased in the hypothalamus of male goldfish. Such differences were not observed in the hypothalamus of female fish, and in the testis of 28 days fasted fish. Meanwhile, preproghrelin, GHSR, and GOAT expression (both mRNA and protein) was significantly increased in the ovary of female fish fasted for 28 days. Ghrelin has been shown to suppress oocyte maturation in fish. The upregulation of a system that has ovarian inbititory roles suggests a role for ghrelin in maintaining reduced reproductive capability during metabolically challenging periods.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10613 - Zoology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Comparative Biochemistry and Physiology A-Molecular &amp; Integrative Physiology

ISSN

1095-6433

e-ISSN

—

Volume of the periodical

235

Issue of the periodical within the volume

neuveden

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

112-120

UT code for WoS article

000481561100011

EID of the result in the Scopus database

2-s2.0-85066464480