Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902503" target="_blank" >RIV/60076658:12520/21:43902503 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/21:00550588
Result on the web
<a href="https://doi.org/10.1007/s12562-020-01481-7" target="_blank" >https://doi.org/10.1007/s12562-020-01481-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12562-020-01481-7" target="_blank" >10.1007/s12562-020-01481-7</a>
Alternative languages
Result language
angličtina
Original language name
Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo
Original language description
The cleavage pattern of a vertebrate's embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the cleavage pattern. To understand the transition, it is essential to develop an effective technique for the inhibition of specific blastomere cleavage. So far, various studies have demonstrated that diatom-derived polyunsaturated aldehyde (PUA), 2,4-decadienal (DD)-a model aldehyde for experimental studies-adversely affects the developing embryos of several aquatic species. In this study, we employed DD for inhibition of cleavage of a definite blastomere in sturgeon embryos under various conditions. The effective treatment was found to be a combination of DD injection (0.01 v/v) and visible light (44.86-91.15 W m(-2)). Notably, DD injection or light irradiation alone cannot inhibit cleavage. Furthermore, spatial RNA localization analysis using quantitative polymerase chain reaction (qPCR)-tomography revealed that the localized pattern of selected maternal messenger ribonucleic acids (mRNAs) remained constant along the animal-vegetal (A-V) axis, which suggests that RNA localization is completed by the end of oogenesis and that early embryonic cleavage is not required for A-V asymmetry preservation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Fisheries Science
ISSN
0919-9268
e-ISSN
1444-2906
Volume of the periodical
87
Issue of the periodical within the volume
1
Country of publishing house
JP - JAPAN
Number of pages
13
Pages from-to
71-83
UT code for WoS article
000604838000001
EID of the result in the Scopus database
2-s2.0-85098769135