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Optimization of sterlet sperm concentration for cryopreservation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43904058" target="_blank" >RIV/60076658:12520/21:43904058 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1016/j.aquaculture.2021.736682" target="_blank" >https://doi.org/10.1016/j.aquaculture.2021.736682</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.aquaculture.2021.736682" target="_blank" >10.1016/j.aquaculture.2021.736682</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Optimization of sterlet sperm concentration for cryopreservation

  • Original language description

    One of the critical points in low temperature storage is the expense of storage capacities. Moreover, sperm concentration in a sample is not only a parameter for effective use of a cryobanks but also a parameter affecting cryopreservation outcomes. Sturgeon sperm in comparison to other species is significantly less concentrated due to the specificity of sperm maturation process, during which sperm is mixed with urine. In this study, we evaluated whether the artificial modification of the sterlet spermatozoa concentration before freezing can be useful during the routine application of sturgeon sperm cryopreservation. The sperm was first concentrated by centrifugation of native sperm samples. Then, the excess of seminal fluid was collected. The concentrated sperm samples were further diluted with the same seminal fluid to obtain different sperm concentrations in suspension, and they were subjected to freeze-thawing. The percentage of postthaw sperm motility depended on the sperm concentration in the samples. The highest post-thaw sperm motility was found in sperm samples with concentration of 0.2 and 1 x 10(9) spz mL(-1). The sperm concentration of 3 x 10(9) spz mL(-1), which is higher than the native sperm concentration in sterlet, was found to be appropriate for use in cryopreservation procedures since the sperm fertilizing ability remained at a high level even though a significant decline in percentage of sperm motility was observed. These findings supported the conclusion that the procedure of artificially increasing the sperm concentration before freezing could be useful for the reduction of the volume kept in cryostorage and to decrease the sample volume required for artificial fertilization.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10604 - Reproductive biology (medical aspects to be 3)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Aquaculture

  • ISSN

    0044-8486

  • e-ISSN

    1873-5622

  • Volume of the periodical

    540

  • Issue of the periodical within the volume

    neuveden

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000649693800001

  • EID of the result in the Scopus database

    2-s2.0-85103737148