High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F23%3A43906200" target="_blank" >RIV/60076658:12520/23:43906200 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2022.738746" target="_blank" >https://doi.org/10.1016/j.aquaculture.2022.738746</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2022.738746" target="_blank" >10.1016/j.aquaculture.2022.738746</a>

Result language

angličtina

Original language name

High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp

Original language description

To avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current study demonstrated that reasonable lowering of dilution rates might still be effective for carp sperm cryobanking &amp; mdash;elevation of spermatozoa con-centration up to 13 x 109 spz mL-1 resulted in a significant decrease in post-thaw sperm motility percentage to 20% compared with 39% in 0.5 x 109 spz mL-1. Nevertheless, no significant differences in sperm kinetic pa-rameters (VCL and LIN) were found in this case. The fertilization outcome (embryo development and hatching rates) was similar after applying thawed sperm samples with &amp; ldquo;optimal &amp; rdquo; (2.2 &amp; ndash;2.4 x 109 spz mL-1) and &amp; ldquo;sub-optimal &amp; rdquo; (11.0 &amp; ndash;13.0 x 109 spz mL-1) concentrations (sperm/egg ratio at fertilization was in the range of 0.3 &amp; ndash;4.5 x 105 spz/egg). Thus, applying a low dilution rate such as one part of sperm to one of the cryoprotective me-diums is favorable for decreasing cryo-storage space and the sperm volume needed to fertilize big egg numbers. The experiment also shows that the 4.5 x 105 spz/egg ratio is not sufficient for good fertilization, and it is necessary to use higher sperm concentrations per egg or improve the method of fertilization. &lt;comment&gt;Superscript/Subscript Available&lt;/comment

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Volume of the periodical

562

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

—

UT code for WoS article

000859629300003

EID of the result in the Scopus database

2-s2.0-85137050969