Identification, Quantification and Kinetic Study of Carotenoids and Lipids in Rhodotorula toruloides CBS 14 Cultivated on Wheat Straw Hydrolysate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904525" target="_blank" >RIV/60076658:12520/22:43904525 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3390/fermentation8070300" target="_blank" >https://doi.org/10.3390/fermentation8070300</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/fermentation8070300" target="_blank" >10.3390/fermentation8070300</a>

Result language

angličtina

Original language name

Identification, Quantification and Kinetic Study of Carotenoids and Lipids in Rhodotorula toruloides CBS 14 Cultivated on Wheat Straw Hydrolysate

Original language description

Production of carotenoids and lipids by Rhodotorula toruloides CBS 14 cultivated on wheat straw hydrolysate was investigated. An ultra-high-performance liquid chromatography (UHPLC) method for carotenoid quantification was developed and validated. Saponification effects on individual carotenoid quantification were identified, and lipid and carotenoid kinetics during cultivation were determined. The carotenoids beta-carotene, gamma-carotene, torularhodin, and torulene were identified; beta-carotene was the major carotenoid, reaching a maximum of 1.48 mg/100 g dry weight. Recoveries of the carotenoids were between 66% and 76%, except torulene and torularhodin, which had lower recoveries due to saponification effects. Total carotenoid content in saponified and unsaponified yeast extract, respectively, determined by UHPLC or photometer, respectively, was 1.99 mg/100 g and 4.02 mg beta-EQ/100 g dry weight. Growth kinetics showed a positive correlation between carotenoid content and lipid accumulation. beta-carotene was the major carotenoid at all time points. At the end of the cultivation, triacylglycerols (TAGs) were the major lipid class, with 58.1% +/- 3.32% of total lipids. There was also a high proportion of free fatty acids, reaching from 20.5% to 41.8% of total lipids. Oleic acid (C18:1) was the major fatty acid. The lipid yield at the end of the cultivation was 0.13 g/g of sugar consumed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20801 - Environmental biotechnology

Project

<a href="/en/project/LM2018099" target="_blank" >LM2018099: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fermentation (Basel)

ISSN

2311-5637

e-ISSN

2311-5637

Volume of the periodical

8

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

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UT code for WoS article

000831856200001

EID of the result in the Scopus database

2-s2.0-85133243846