Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F02%3A00005772" target="_blank" >RIV/60076658:12610/02:00005772 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula)

Original language description

Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1 then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100 % cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full peri

Czech name

Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula)

Czech description

Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1 then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100 % cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full peri

Type

D - Article in proceedings

CEP classification

GL - Fishery

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

V. Česká ichtyologická konference, Sborník referátů z vědecké konference

ISBN

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ISSN

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e-ISSN

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Number of pages

2

Pages from-to

326-328

Publisher name

MZLU Brno

Place of publication

Brno

Event location

Brno

Event date

Jan 1, 2002

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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