Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004199" target="_blank" >RIV/60076658:_____/02:00004199 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
čeština
Original language name
Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula)
Original language description
Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1 then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100 % cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full peri
Czech name
Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula)
Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GL - Fishery
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
V. Česká ichtyologická konference, Sborník referátů z vědecké konference
ISBN
80-7157-614-X
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
326-328
Publisher name
MZLU Brno
Place of publication
Brno
Event location
Brno
Event date
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Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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