Accumulation of the D2 protein is a key regulatory step for assembly of the photosystem II reaction center complex in Synechocystis PCC 6803

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F04%3A00005369" target="_blank" >RIV/60076658:12640/04:00005369 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/04:00108075

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Accumulation of the D2 protein is a key regulatory step for assembly of the photosystem II reaction center complex in Synechocystis PCC 6803

Original language description

Accumulation of monomer and dimer photosystem (PS) II reaction center core complexes has been analyzed by two-dimensional Blue-native/ SDS-PAGE in Synechocystis PCC 6803 wild type and in mutant strains lacking genes psbA, psbB, psbC, psbDIC/DII, or the psbEFLJ operon. In vivo pulse-chase radiolabeling experiments revealed that mutant cells assembled PSII precomplexes only. In DeltapsbC and DeltapsbB, assembly of reaction center cores lacking CP43 and reaction center complexes was detected, respectively.In DeltapsbA, protein subunits CP43, CP47, D2, and cytochrome b(559) were synthesized, but proteins did not assemble. Similarly, in DeltapsbD/C lacking D2, and CP43, the de novo synthesized proteins D1, CP47, and cytochrome b559 did not form any mutualcomplexes, indicating that assembly of the reaction center complex is a prerequisite for assembly with core subunits CP47 and CP43. Finally, although CP43 and CP47 accumulated in DeltapsbEFLJ, D2 was neither expressed nor accumulated. We,

Czech name

Akumulace bílkoviny D2 je klíčovým regulačním krokem v sestavování komplexu reakčního centra fotosystému II v Synechocystis PCC 6803

Czech description

Akumulace komplexů fotosystému II byla studována kombinací nativní a denaturační elektroforézy v divokém kmeni sinice Synechocystis sp. PCC 6803 a jejích mutantech s chybějícími geny kódujícími proteiny CP43, CP47, D2, D1 a cytochrom b-559. Radioaktivníznačení ukázalo, že mutanti nejsou schopni skládat celé komplexy PSII. V kmeni bez proteinu CP43 se akumulovalo jádro komplexu bez CP43, zatímco v kmeni bez CP47 byly nalezeny komplexy reakčního centra a volný CP43. V kmenech s chybějícími proteiny D1 nebo D2 byly ostatní proteiny syntetizovány, ale netvořily žádné společné komplexy. V kmeni bez cytochromu b-559 byla téměř zastavena syntéza D2 a tak se kmen podobal kmeni bez proteinu D2 a rovněž nebyl schopen skládat žádné komplexy mezi proteiny fotosystému.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/LN00A141" target="_blank" >LN00A141: Mechanism, ecophysiology and biotechnology of photosynthesis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

279

Issue of the periodical within the volume

47

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

48620-48629

UT code for WoS article

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EID of the result in the Scopus database

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