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Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004197" target="_blank" >RIV/60076658:_____/02:00004197 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12610/02:00005771

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility

  • Original language description

    The main aims of the present work were to examine effects of potassium, calcium and immobilizing medium on motility of fresh and demembranated spermatozoa of paddlefish (Polyodon spathula). Evaluation of sperm motility parameters Sperm motility was evaluated for the percentage of motile spermatozoa from video frames, velocity was measured as the distance covered by sperm heads during the corresponding to three video frames and frequency was measured using dark-field microscopy coupled with stroboscopicillumination. Short-term storage of paddlefish sperm in immobilizing medium Seminal plasma of paddlefish has a low spermatozoa concentration (around 0.1-1x109 sperm), therefore it was necessary to concentrate cells by centrifugation (2000 g for 5 min). For examination, 100 #l of centrifuged seminal fluid (transparent part) was diluted with following immobilizing solution (IM): 900#l of seminal fluid, 800 #l of seminal fluid and 100 #l DMSO, 900#l of SM containing 5 mM KCl, 800 #l of SM c

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    GL - Fishery

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2002

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    XXth Genetics Days (Proceedings of the Intenational Scientifics Conference

  • ISBN

    80-7157-607-7

  • ISSN

  • e-ISSN

  • Number of pages

    2

  • Pages from-to

    322-323

  • Publisher name

    Mendelova zemědělská a lesnická univerzita

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Sep 12, 2002

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article