Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004197" target="_blank" >RIV/60076658:_____/02:00004197 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12610/02:00005771
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility
Original language description
The main aims of the present work were to examine effects of potassium, calcium and immobilizing medium on motility of fresh and demembranated spermatozoa of paddlefish (Polyodon spathula). Evaluation of sperm motility parameters Sperm motility was evaluated for the percentage of motile spermatozoa from video frames, velocity was measured as the distance covered by sperm heads during the corresponding to three video frames and frequency was measured using dark-field microscopy coupled with stroboscopicillumination. Short-term storage of paddlefish sperm in immobilizing medium Seminal plasma of paddlefish has a low spermatozoa concentration (around 0.1-1x109 sperm), therefore it was necessary to concentrate cells by centrifugation (2000 g for 5 min). For examination, 100 #l of centrifuged seminal fluid (transparent part) was diluted with following immobilizing solution (IM): 900#l of seminal fluid, 800 #l of seminal fluid and 100 #l DMSO, 900#l of SM containing 5 mM KCl, 800 #l of SM c
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GL - Fishery
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XXth Genetics Days (Proceedings of the Intenational Scientifics Conference
ISBN
80-7157-607-7
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
322-323
Publisher name
Mendelova zemědělská a lesnická univerzita
Place of publication
Brno
Event location
Brno
Event date
Sep 12, 2002
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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