Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334909" target="_blank" >RIV/60077344:_____/09:00334909 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/09:00010578

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase

Original language description

The mitochondrial RNA binding complex 1 (MRB1) is a recently discovered set of proteins associated with the TbRGG1 in Trypanosoma brucei. Based on the phenotype caused by downregulation of this protein, it was proposed to play an unspecified RNA editingrole. RNAi-silencing of three newly characterized protein subunits, guide RNA associated proteins (GAPs) 1 and 2 plus a predicted RNA helicase, show they are essential for cell growth in the insect stage. Furthermore, their downregulation leads to inhibition of editing in only those mRNAs for which minicircle-encoded guide (g) RNAs are required. All three proteins are necessary for the expression of the minicircle-encoded gRNAs. Moreover, down-regulation of a fourth assayed putative MRB1 subunit, Nudixhydrolase, does not appear to destabilize gRNAs but has a general impact on the stability of maxicircle-encoded RNAs. We also demonstrate that the same mt RNA polymerase may also have a role in expression of the minicircle-encoded gRNAs.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

R N A

ISSN

1355-8382

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

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UT code for WoS article

00264280100008

EID of the result in the Scopus database

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