Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00347925" target="_blank" >RIV/60077344:_____/10:00347925 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

Original language description

The absence of a general gene-targeting system for systematic reverse genetic studies has been a significant limitation in silkworm research. The authors performed direct zinc finger nuclease mRNA injection to /B. mori/ embryos and induced mutations in an epidermal color gene /BmBLOS2. /The results suggest that the /B. mori/ double-strand break repair system relies on microhomologies rather than on a ligase IV-dependent mechanism typical for /Drosophila/. The observed frequency of germline mutations wassufficient for future use in gene targeting relying solely on molecular methods of screening.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Insect Biochemistry and Molecular Biology

ISSN

0965-1748

e-ISSN

—

Volume of the periodical

40

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

—

UT code for WoS article

000283390300007

EID of the result in the Scopus database

—