Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00349395" target="_blank" >RIV/60077344:_____/10:00349395 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/10:00349395 RIV/61388963:_____/10:00349395

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus

Original language description

IRS-2 from the hard tick Ixodes ricinus belongs into the serpin family of protease inhibitors. It is produced in the tick salivary glands, and its anti-inflammatory activity suggests a role in the parasitehost interaction. Recombinant IRS-2 prepared by heterologous expression in bacterial system was crystallized and crystals diffracted to resolution 1.8 ?. IRS-2 was cleaved during crystallization by contaminating proteases. This processing of IRS-2 mimicked the specific cleavage of serpin by its targetprotease and resulted in a more stable R conformation, which produced well-diffracting crystals. Activity profiling with specific substrates and inhibitors demonstrated traces of serine and cysteine proteases in the protein stock solution.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Crystallographica Section F

ISSN

1744-3091

e-ISSN

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Volume of the periodical

F66

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

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UT code for WoS article

000283714100011

EID of the result in the Scopus database

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