MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00359407" target="_blank" >RIV/60077344:_____/11:00359407 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/11:43880918
Result on the web
<a href="http://dx.doi.org/10.1261/rna.2446311" target="_blank" >http://dx.doi.org/10.1261/rna.2446311</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.2446311" target="_blank" >10.1261/rna.2446311</a>
Alternative languages
Result language
angličtina
Original language name
MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process
Original language description
In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
R N A
ISSN
1355-8382
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
865-877
UT code for WoS article
000289617400009
EID of the result in the Scopus database
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