Architecture of the trypanosome RNA editing accessory complex, MRB1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381882" target="_blank" >RIV/60077344:_____/12:00381882 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/12:43883517

Result on the web

<a href="http://nar.oxfordjournals.org/content/40/12/5637.full" target="_blank" >http://nar.oxfordjournals.org/content/40/12/5637.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks211" target="_blank" >10.1093/nar/gks211</a>

Result language

angličtina

Original language name

Architecture of the trypanosome RNA editing accessory complex, MRB1

Original language description

Trypanosoma brucei undergoes an essential process of mitochondrial uridine insertion and deletion RNA editing catalyzed by a 20S editosome. The multiprotein mitochondrial RNA-binding complex 1 (MRB1) is emerging as an equally essential component of the trypanosome RNA editing machinery, with additional functions in gRNA and mRNA stabilization. The distinct and overlapping protein compositions of reported MRB1 complexes and diverse MRB1 functions suggest that the complex is composed of subcomplexes withRNA-dependent and independent interactions. To determine the architecture of the MRB1 complex, we performed a comprehensive yeast two-hybrid analysis of 31 reported MRB1 proteins. We also used in vivo analyses of tagged MRB1 components to confirm directand RNA-mediated interactions. Here, we show that MRB1 contains a core complex comprised of six proteins and maintained by numerous direct interactions. The MRB1 core associates with multiple subcomplexes and proteins through RNA-enhanced

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F09%2F1667" target="_blank" >GA204/09/1667: Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

5637-5650

UT code for WoS article

000305829000046

EID of the result in the Scopus database

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