Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography?mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00376118" target="_blank" >RIV/60077344:_____/12:00376118 - isvavai.cz</a>

Alternative codes found

RIV/00843989:_____/12:00102564

Result on the web

<a href="http://www.springerlink.com/content/781wln3085q3v21r/" target="_blank" >http://www.springerlink.com/content/781wln3085q3v21r/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-012-5727-y" target="_blank" >10.1007/s00216-012-5727-y</a>

Result language

angličtina

Original language name

Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography?mass spectrometry

Original language description

Four disulfide-reducing agents, dithiothreitol (DTT), 2,3-dimercaptopropanesulfonate (DMPS), and the newly tested 2-mercaptoethanesulfonate (MESNA) and tris(hydroxypropyl)phosphine (THP), were investigated in detail for release of sulfur amino acids in human plasma. After protein precipitation with trichloroacetic acid (TCA), the plasma supernatant was treated with methyl, ethyl, or propyl chloroformate and the products were subjected to GC?MS analysis. All the tested agents proved to be rapid and effective reducing agents for the assay of plasma thiols. When compared with DTT, the novel reducing agents DMPS, MESNA, and THP provided much cleaner extracts and improved analytical performance. Validation performed with the THP agent and propyl chloroformate derivatization demonstrated the robustness and reliability of this simple sample-preparation methodology.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

402

Issue of the periodical within the volume

9

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

2953-2963

UT code for WoS article

000301178000025

EID of the result in the Scopus database

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