Laboratory bioassay assessing the enhancement of Cry3Aa toxicity to coleopteran storage pests ? preliminary results
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00423244" target="_blank" >RIV/60077344:_____/13:00423244 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Laboratory bioassay assessing the enhancement of Cry3Aa toxicity to coleopteran storage pests ? preliminary results
Original language description
Storage pests cause economic losses to stored grain worldwide. The traditional synthetic chemicals that have been used for their control are increasingly removed from the market because of increased regulatory constraints and insect resistance. The use of biopesticides based on Bacillus thuringiensis (Bt) spores offers a safe alternative but some storage pests show low sensitivity to any natural Cry toxin. Several recombinant proteins with N-/C-terminal and binding domain modifications of Cry3Aa were prepared in search for a derivative active on Tenebrio molitor and Tribolium castaneum (Coleoptera: Tenebrionidae). Test compounds were added to the standard larval diet and larval mortality was recorded in seven-day intervals for eight weeks of exposure.Probit analysis was used to estimate lethal effects of four doses of Cry3Aa and its derivatives and the LC50 was calculated. Slightly enhanced effects of some Cry3Aa derivatives in comparison with natural Cry3Aa toxin were detected.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP502%2F11%2F1471" target="_blank" >GAP502/11/1471: Study of mechanisms of insect pests resistance against Bacillus thuringiensis Cry toxins</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
IOBC-WPRS Bulletin (GMOs in Integrated Plant Production)
ISBN
978-92-9067-276-0
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
109-113
Publisher name
Federal Research Center for Cultivated Plants
Place of publication
Darmstadt
Event location
Berlín
Event date
Jun 3, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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