Fragments of Tenebrio molitor cadherin enhance Cry3Aa toxicity for the red flour beetle, Tribolium castaneum (Herbst)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00464839" target="_blank" >RIV/60077344:_____/16:00464839 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/jen.12255" target="_blank" >http://dx.doi.org/10.1111/jen.12255</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jen.12255" target="_blank" >10.1111/jen.12255</a>

Result language

angličtina

Original language name

Fragments of Tenebrio molitor cadherin enhance Cry3Aa toxicity for the red flour beetle, Tribolium castaneum (Herbst)

Original language description

Bacillus thuringiensis crystalline (Cry) proteins are highly toxic to a wide range of insect pests, but some species resist their action. This is true for many economically important beetles, including stored product pests, such as Tribolium castaneum. In this article, we show that the susceptibility of T.castaneum larvae to natural as well as to a recombinant Cry3Aa-type toxin, applied in the diet, is enhanced by supplementing the diet with recombinant fragments of Tenebrio molitor cadherin; Cry toxin-binding sites occur in several cadherin repeats (CR). In our study, we used the toxin-binding region CRtb, which represents a substantial part of the repeat CR12-MPED (membrane-proximal extracellular domain). CRtb and CR12-MPED consistently increased Cry3Aa toxicity. This synergistic effect occured at diverse mass ratios between the toxin and the cadherin fragments, suggesting that optimal ratios can be found. In our 6-week-long assay with T.castaneum, we achieved mortality of up to 96.6% with toxin concentration 30g/g. Cadherin fragments CR11 and CR9-11 elicited small and diverse effects that require further analysis.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Entomology

ISSN

0931-2048

e-ISSN

—

Volume of the periodical

140

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

277-286

UT code for WoS article

000374494400005

EID of the result in the Scopus database

2-s2.0-84937597690