RAP-PCR fingerprinting reveals time-dependent expression of development-related genes following differentiation process of Bacillus thuringiensis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00447104" target="_blank" >RIV/60077344:_____/15:00447104 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1139/cjm-2015-0212" target="_blank" >http://dx.doi.org/10.1139/cjm-2015-0212</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/cjm-2015-0212" target="_blank" >10.1139/cjm-2015-0212</a>
Alternative languages
Result language
angličtina
Original language name
RAP-PCR fingerprinting reveals time-dependent expression of development-related genes following differentiation process of Bacillus thuringiensis
Original language description
Gene expression profiles are important data to reveal the functions of genes putatively involved in crucial biological processes. RNA arbitrarily primed polymerase chain reaction (RAP-PCR) and specifically primed reverse transcription polymerase chain reaction (RT-PCR) were combined to screen differentially expressed genes following development of a commercial Bacillus thuringiensis subsp. kurstaki strain 8010 (serotype 3a3b). Six differentially expressed transcripts (RAP1 to RAP6) were obtained. RAP1 encoded a putative triple helix repeat-containing collagen or an exosporium protein H related to spore pathogenicity. RAP2 was homologous to a ClpX protease and an ATP-dependent protease La (LonB), which likely acted as virulence factors. RAP3 was homologous to a beta subunit of propionyl-CoA carboxylase required for the development of Myxococcus xanthus. RAP4 had homology to a quinone oxidoreductase involved in electron transport and ATP formation. RAP5 showed significant homology to a u
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Canadian Journal of Microbiology
ISSN
0008-4166
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
9
Country of publishing house
CA - CANADA
Number of pages
8
Pages from-to
683-690
UT code for WoS article
000360946800009
EID of the result in the Scopus database
2-s2.0-84944559152