Expression profiling of circulating tumor cells in metastatic breast cancer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00450240" target="_blank" >RIV/60077344:_____/15:00450240 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10549-014-3215-0" target="_blank" >http://dx.doi.org/10.1007/s10549-014-3215-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10549-014-3215-0" target="_blank" >10.1007/s10549-014-3215-0</a>
Alternative languages
Result language
angličtina
Original language name
Expression profiling of circulating tumor cells in metastatic breast cancer
Original language description
Circulating tumor cells (CTCs) are prognostic in all stages of breast cancer. However, since they are extremely rare, little is known about the molecular nature of these cells. We report a novel strategy for the isolation and expression profiling of purepopulations of CTCs derived from peripheral blood. We developed a method to isolate CTCs based on immunomagnetic capture followed by fluorescence-activated cell sorting (IE/FACS). After assay validation using the BT474 cell line spiked into blood samples in vitro, RNA from CTCs isolated from the blood of five metastatic breast cancer (MBC) patients was linearly amplified and subjected to gene expression profiling via cDNA microarrays. We isolated a range of 9-993 captured CTCs from five MBC patients' blood and profiled their RNA in comparison to a diverse panel of primary breast tumors (n = 55). Differential expression analysis revealed CTCs to have downregulated apoptosis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Breast Cancer Research and Treatment
ISSN
0167-6806
e-ISSN
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Volume of the periodical
149
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
121-131
UT code for WoS article
000348183700013
EID of the result in the Scopus database
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