Computational exploration of microRNAs from expressed sequence tags of Humulus lupulus, target predictions and expression analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00450271" target="_blank" >RIV/60077344:_____/15:00450271 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.compbiolchem.2015.09.005" target="_blank" >http://dx.doi.org/10.1016/j.compbiolchem.2015.09.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.compbiolchem.2015.09.005" target="_blank" >10.1016/j.compbiolchem.2015.09.005</a>

Result language
angličtina
Original language name
Computational exploration of microRNAs from expressed sequence tags of Humulus lupulus, target predictions and expression analysis
Original language description
Among computationally predicted and experimentally validated plant miRNAs, several are conserved across species boundaries in the plant kingdom. In this study, a combined experimental-in silico computational based approach was adopted for the identification and characterization of miRNAs in Humulus lupulus (hop), which is widely cultivated for use by the brewing industry and apart from, used as a medicinal herb. A total of 22 miRNAs belonging to 17 miRNA families were identified in hop following comparative computational approach and EST-based homology search according to a series of filtering criteria. Selected miRNAs were validated by end-point PCR and quantitative reverse transcription- polymerase chain reaction (qRT-PCR), confirmed the existence ofconserved miRNAs in hop. Based on the characteristic that miRNAs exhibit perfect or nearly perfect complementarity with their targeted mRNA sequences, a total of 47 potential miRNA targets were identified in hop.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GA13-03037S" target="_blank" >GA13-03037S: Combinatorial regulation and network of transcription factors involved in the biosynthesis of medicinal prenylflavonoids in hop (Humulus lupulus L.).</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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