Centromeric and non-centromeric satellite DNA organisation differs in holocentric Rhynchospora species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00464891" target="_blank" >RIV/60077344:_____/17:00464891 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00412-016-0616-3" target="_blank" >http://dx.doi.org/10.1007/s00412-016-0616-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00412-016-0616-3" target="_blank" >10.1007/s00412-016-0616-3</a>
Alternative languages
Result language
angličtina
Original language name
Centromeric and non-centromeric satellite DNA organisation differs in holocentric Rhynchospora species
Original language description
Satellite DNA repeats (or satDNA) are fast-evolving sequences usually associated with condensed heterochromatin. To test whether the chromosomal organisation of centromeric and non-centromeric satDNA differs in species with holocentric chromosomes, we identified and characterised the major satDNA families in the holocentric Cyperaceae species Rhynchospora ciliata (2n = 10), R. globosa (2n = 50) and R. tenuis (2n = 2x = 4 and 2n = 4x = 8). While conserved centromeric repeats (present in R. ciliata and R. tenuis) revealed linear signals at both chromatids, non-centromeric, species-specific satDNAs formed distinct clusters along the chromosomes. Colocalisation of both repeat types resulted in a ladder-like hybridisation pattern at mitotic chromosomes. In interphase, the centromeric satDNA was dispersed while non-centromeric satDNA clustered and partly colocalised to chromocentres. Despite the banding-like hybridisation patterns of the clustered satDNA, the identification of chromosome pairs was impaired due to the irregular hybridisation patterns of the homologues in R. tenuis and R. ciliata. These differences are probably caused by restricted or impaired meiotic recombination as reported for R. tenuis, or alternatively by complex chromosome rearrangements or unequal condensation of homologous metaphase chromosomes. Thus, holocentricity influences the chromosomal organisation leading to differences in the distribution patterns and condensation dynamics of centromeric and non-centromeric satDNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/GBP501%2F12%2FG090" target="_blank" >GBP501/12/G090: Evolution and Function of Complex Plant Genomes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chromosoma
ISSN
0009-5915
e-ISSN
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Volume of the periodical
126
Issue of the periodical within the volume
September 19
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
325-335
UT code for WoS article
000397951000010
EID of the result in the Scopus database
2-s2.0-84988431922