RSM22, mtYsxC and PNKD-like proteins are required for mitochondrial translation in Trypanosoma brucei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00479262" target="_blank" >RIV/60077344:_____/17:00479262 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/17:43895457

Result on the web

<a href="http://dx.doi.org/10.1016/j.mito.2017.01.003" target="_blank" >http://dx.doi.org/10.1016/j.mito.2017.01.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mito.2017.01.003" target="_blank" >10.1016/j.mito.2017.01.003</a>

Result language

angličtina

Original language name

RSM22, mtYsxC and PNKD-like proteins are required for mitochondrial translation in Trypanosoma brucei

Original language description

Mitochondrial ribosomes evolved from prokaryotic ribosomes, with which they therefore share more common features than with their counterparts in the cytosol. Yet, mitochondrial ribosomes are highly diverse in structure and composition, having undergone considerable changes, including reduction of their RNA component and varying degree of acquisition of novel proteins in various phylogenetic lineages. Here, we present functional analysis of three putative mitochondrial ribosome-associated proteins (RSM22, mtYsxC and PNKD-like) in Trypanosoma brucei, originally identified by database mining. While in other systems the homologs of RSM22 are known as components of mitochondria ribosomes, YsxC was linked with ribosomes only in bacteria. The PNKD-like protein shows similarity to a human protein, the defects of which cause PNKD (paroxysmal nonkinesigenic dyskinesia). Here we show that all three proteins are important for the growth of T. brucei. They play an important function in mitochondrial translation, as their ablation by RNAi rapidly and severely affected the de novo synthesis of mitochondrial proteins. Moreover, following the RNAi-mediated depletion of RSM22, structure of the small subunit of mitochondrial ribosome becomes severely compromised, suggesting a role of RSM22 in ribosomal assembly and/or stability. (C) 2017 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/GA15-21974S" target="_blank" >GA15-21974S: Mitochondrial genome-wide studies of RNA-binding proteins in trypanosomes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mitochondrion

ISSN

1567-7249

e-ISSN

—

Volume of the periodical

34

Issue of the periodical within the volume

MAY

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

67-74

UT code for WoS article

000402023900010

EID of the result in the Scopus database

2-s2.0-85009952092