SUMOylated SNF2PH promotes variant surface glycoprotein expression in bloodstream trypanosomes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00520462" target="_blank" >RIV/60077344:_____/19:00520462 - isvavai.cz</a>
Result on the web
<a href="https://www.embopress.org/doi/pdf/10.15252/embr.201948029" target="_blank" >https://www.embopress.org/doi/pdf/10.15252/embr.201948029</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embr.201948029" target="_blank" >10.15252/embr.201948029</a>
Alternative languages
Result language
angličtina
Original language name
SUMOylated SNF2PH promotes variant surface glycoprotein expression in bloodstream trypanosomes
Original language description
SUMOylation is a post-translational modification that positively regulates monoallelic expression of the trypanosome variant surface glycoprotein (VSG). The presence of a highly SUMOylated focus associated with the nuclear body, where the VSG gene is transcribed, further suggests an important role of SUMOylation in regulating VSG expression. Here, we show that SNF2PH, a SUMOylated plant homeodomain (PH)-transcription factor, is upregulated in the bloodstream form of the parasite and enriched at the active VSG telomere. SUMOylation promotes the recruitment of SNF2PH to the VSG promoter, where it is required to maintain RNA polymerase I and thus to regulate VSG transcript levels. Further, ectopic overexpression of SNF2PH in insect forms, but not of a mutant lacking the PH domain, induces the expression of bloodstream stage-specific surface proteins. These data suggest that SNF2PH SUMOylation positively regulates VSG monoallelic transcription, while the PH domain is required for the expression of bloodstream-specific surface proteins. Thus, SNF2PH functions as a positive activator, linking expression of infective form surface proteins and VSG regulation, thereby acting as a major regulator of pathogenicity.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO REPORTS
ISSN
1469-3178
e-ISSN
—
Volume of the periodical
NOV 2019
Issue of the periodical within the volume
NOV 2019
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
e48029
UT code for WoS article
000494626400001
EID of the result in the Scopus database
2-s2.0-85074820721