SubtypingCryptosporidium ryanae: A Common Pathogen in Bovine Animals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00538194" target="_blank" >RIV/60077344:_____/20:00538194 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2076-2607/8/8/1107" target="_blank" >https://www.mdpi.com/2076-2607/8/8/1107</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms8081107" target="_blank" >10.3390/microorganisms8081107</a>

Result language

angličtina

Original language name

SubtypingCryptosporidium ryanae: A Common Pathogen in Bovine Animals

Original language description

Cryptosporidium ryanaeis one of the most common species for cryptosporidiosis in cattle. However, little is known of the genetic characteristics ofC. ryanaedue to the lack of subtyping tools. In the present study, the 60-kDa glycoprotein (gp60) gene ofC. ryanaewas identified in whole genome sequence data and analyzed for sequence characteristics using bioinformatics tools. The protein it encodes had some of the typical characteristics of GP60 proteins, with a signal peptide, a furin cleavage site, and a glycosylphosphatidylinositol anchor at the C terminus of the protein, and numerous O-glycosylation sites. The gene sequence was used in the development of a subtyping tool, which was used in characterizingC. ryanaefrom 110 specimens from dairy cattle, 2 from beef cattle, 6 from yaks, and 4 from water buffaloes in China. Altogether, 17 subtypes from 8 subtype families were recognized, namely XXIa to XXIh. Possible host adaption was identified within this species, reflected by the unique occurrence of XXIa, XXIc, and XXIh in dairy cattle, yaks, and water buffaloes, respectively. Some geographical differences were detected in the distribution of subtype families in dairy cattle, specimens from southern China showed higher genetic diversity than from northern China, and the XXIa subtype family was only seen in dairy cattle in southern and eastern China. Thegp60-based subtyping tool should be useful in molecular epidemiological studies of the transmission ofC. ryanae.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microorganisms

ISSN

2076-2607

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

1107

UT code for WoS article

000564827900001

EID of the result in the Scopus database

2-s2.0-85088567403