Biochemically identified neuropeptides in a caddisfly (Trichoptera) and a pygmy mole cricket (Orthoptera: Caelifera: Tridactyloidea)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00541151" target="_blank" >RIV/60077344:_____/21:00541151 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/arch.21778" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/arch.21778</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/arch.21778" target="_blank" >10.1002/arch.21778</a>
Alternative languages
Result language
angličtina
Original language name
Biochemically identified neuropeptides in a caddisfly (Trichoptera) and a pygmy mole cricket (Orthoptera: Caelifera: Tridactyloidea)
Original language description
One representative of the order Trichoptera, namely the caddisfly Chaetopteryx villosa, was investigated along with the pygmy mole cricket Xya capensis which is a representative of the most basal superfamily of the caeliferan Orthoptera, that is, the Tridactyloidea. From both clades neuropeptides have not been biochemically characterized before this study. Here, members of the adipokinetic hormone family (AKHs) are sequenced via liquid chromatography (LC)‐ion trap mass spectrometry from methanolic extracts from the corpora cardiaca of respective species. The corpora cardiaca were dissected, methanolic extracts prepared, peptides separated by liquid chromatography (LC), and AKHs detected and sequenced by ion trap mass spectrometry. Both species investigated contain an octapeptide AKH: the trichopteran species has the peptide with the sequence pGlu‐Leu‐Thr‐Phe‐Thr‐Pro‐ Ser‐Trp amide, the ambiguity of the isobaric amino acids Leu and Ile at position two was solved by comparing retention times on LC and by co‐elution with the synthetic Leu2‐form. This peptide is known as Aedae‐AKH and found in certain dipteran species and in an alderfly (Megaloptera). The tridactyloid species contains the peptide with the sequence pGlu‐Val‐Asn‐Phe‐Ser‐Pro‐Gly‐Trp amide which had first been identified in a member of the order Mantophasmatodea and is called Manto‐CC. Comparisons are made between the AKH complements of the sister groups Trichoptera and Lepidoptera and their possible relatedness and, on the other hand, between the AKH of X. capensis with those of closely related caeliferan superfamilies. The biology of the two studied species is used to speculate about a possible function of the elucidated hormones. Lastly, the use of a larval stage as starting material for structural neuropeptide information is discussed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA17-22276S" target="_blank" >GA17-22276S: New methods for metabolomic analysis of hardly determined metabolites</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Insect Biochemistry and Physiology
ISSN
0739-4462
e-ISSN
1520-6327
Volume of the periodical
106
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
e21778
UT code for WoS article
000628861700001
EID of the result in the Scopus database
2-s2.0-85102472943