Quantitative proteomics analysis reveals core and variable tick salivary proteins at the tick-vertebrate host interface

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00560034" target="_blank" >RIV/60077344:_____/22:00560034 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/22:43905103

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/mec.16561" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/mec.16561</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/mec.16561" target="_blank" >10.1111/mec.16561</a>

Result language

angličtina

Original language name

Quantitative proteomics analysis reveals core and variable tick salivary proteins at the tick-vertebrate host interface

Original language description

Few studies have examined tick proteomes, how they adapt to their environment, and their roles in the parasite-host interactions that drive tick infestation and pathogen transmission. Here we used a proteomics approach to screen for biologically and immunologically relevant proteins acting at the tick-host interface during tick feeding and, as proof of principle, measured host antibody responses to some of the discovered candidates. We used a label-free quantitative proteomic workflow to study salivary proteomes of (i) wild Ixodes ricinus ticks fed on different hosts, (ii) wild or laboratory ticks fed on the same host, and (iii) adult ticks cofed with nymphs. Our results reveal high and stable expression of several protease inhibitors and other tick-specific proteins under different feeding conditions. Most pathways functionally enriched in sialoproteomes were related to proteolysis, endopeptidase, and amine-binding activities. The generated catalogue of tick salivary proteins enabled the selection of six candidate secreted immunogenic peptides for rabbit immunizations, three of which induced strong and durable antigen-specific antibody responses in rabbits. Furthermore, rabbits exposed to ticks mounted immune responses against the candidate peptides/proteins, confirming their expression at the tick-vertebrate interface. Our approach provides insights into tick adaptation strategies to different feeding conditions and promising candidates for developing antitick vaccines or markers of exposure of vertebrate hosts to tick bites.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Ecology

ISSN

0962-1083

e-ISSN

1365-294X

Volume of the periodical

31

Issue of the periodical within the volume

15

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

4162-4175

UT code for WoS article

000812724100001

EID of the result in the Scopus database

2-s2.0-85132117128