Identification of the Telomere elongation Mutation in Drosophila
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00563932" target="_blank" >RIV/60077344:_____/22:00563932 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905332
Result on the web
<a href="https://www.mdpi.com/2073-4409/11/21/3484" target="_blank" >https://www.mdpi.com/2073-4409/11/21/3484</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cells11213484" target="_blank" >10.3390/cells11213484</a>
Alternative languages
Result language
angličtina
Original language name
Identification of the Telomere elongation Mutation in Drosophila
Original language description
Telomeres in Drosophila melanogaster, which have inspired a large part of Sergio Pimpinelli work, are similar to those of other eukaryotes in terms of their function. Yet, their length maintenance relies on the transposition of the specialized retrotransposons Het-A, TART, and TAHRE, rather than on the activity of the enzyme telomerase as it occurs in most other eukaryotic organisms. The length of the telomeres in Drosophila thus depends on the number of copies of these transposable elements. Our previous work has led to the isolation of a dominant mutation, Tel1, that caused a several-fold elongation of telomeres. In this study, we molecularly identified the Tel1 mutation by a combination of transposon-induced, site-specific recombination and next-generation sequencing. Recombination located Tel1 to a 15 kb region in 92A. Comparison of the DNA sequence in this region with the Drosophila Genetic Reference Panel of wild-type genomic sequences delimited Tel1 to a 3 bp deletion inside intron 8 of Ino80. Furthermore, CRISPR/Cas9-induced deletions surrounding the same region exhibited the Tel1 telomere phenotype, confirming a strict requirement of this intron 8 gene sequence for a proper regulation of Drosophila telomere length.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cells
ISSN
2073-4409
e-ISSN
2073-4409
Volume of the periodical
11
Issue of the periodical within the volume
21
Country of publishing house
CH - SWITZERLAND
Number of pages
18
Pages from-to
3484
UT code for WoS article
000884536200001
EID of the result in the Scopus database
2-s2.0-85141589970