AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form <i>Trypanosoma brucei</i> parasites

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00579861" target="_blank" >RIV/60077344:_____/23:00579861 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/jeu.12994" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/jeu.12994</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jeu.12994" target="_blank" >10.1111/jeu.12994</a>

Result language

angličtina

Original language name

AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form <i>Trypanosoma brucei</i> parasites

Original language description

Selection and internalization of cargo via clathrin-mediated endocytosis requires adaptor protein complexes. One complex, AP-2, acts during cargo selection at the plasma membrane. African trypanosomes lack all components of the AP-2 complex, except for a recently identified orthologue of the AP-2-associated protein kinase 1, AAK1. In characterized eukaryotes, AAK1 phosphorylates the mu 2 subunit of the AP-2 complex to enhance cargo recognition and uptake into clathrin-coated vesicles. Here, we show that kinetoplastids encode not one, but two AAK1 orthologues: one (AAK1L2) is absent from salivarian trypanosomes, while the other (AAK1L1) lacks important kinase-specific residues in a range of trypanosomes. These AAK1L1 and AAK1L2 novelties reinforce suggestions of functional divergence in endocytic uptake within salivarian trypanosomes. Despite this, we show that AAK1L1 null mutant Trypanosoma brucei, while viable, display slowed proliferation, morphological abnormalities including swelling of the flagellar pocket, and altered cargo uptake. In summary, our data suggest an unconventional role for a putative pseudokinase during endocytosis and/or vesicular trafficking in T. brucei, independent of AP-2.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Eukaryotic Microbiology

ISSN

1066-5234

e-ISSN

1550-7408

Volume of the periodical

70

Issue of the periodical within the volume

DEC

Country of publishing house

US - UNITED STATES

Number of pages

20

Pages from-to

e12994

UT code for WoS article

001043849700001

EID of the result in the Scopus database

2-s2.0-85167344778