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Melittin—The principal toxin of honeybee venom—Is also produced in the honeybee fat body

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00585498" target="_blank" >RIV/60077344:_____/24:00585498 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/24:43907982 RIV/60076658:12520/24:43907982

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S1532045624000966/pdfft?md5=3664f989524bf5def364284a6ad5b78b&pid=1-s2.0-S1532045624000966-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1532045624000966/pdfft?md5=3664f989524bf5def364284a6ad5b78b&pid=1-s2.0-S1532045624000966-main.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.cbpc.2024.109928" target="_blank" >10.1016/j.cbpc.2024.109928</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Melittin—The principal toxin of honeybee venom—Is also produced in the honeybee fat body

  • Original language description

    Melittin is a powerful toxin present in honeybee venom that is active in a wide range of animals, from insects to humans. Melittin exerts numerous biological, toxicological, and pharmacological effects, the most important of which is destruction of the cell membrane. The phospholipase activity of melittin and its ability to activate phospholipases in the venom contribute to these actions. Using analytical methods, we discovered that the honeybee Apis mellifera produces melittin not only in the venom gland but also in its fat body cells, which remain resistant to this toxin's effects. We suggest that melittin acts as an anti-bacterial agent, since its gene expression is significantly upregulated when honeybees are infected with Escherichia coli and Listeria monocytogenes bacteria, additionally, melittin effectively kills these bacteria in the disc diffusion test. We hypothesize that the chemical and physicochemical properties of the melittin molecule (hydrophilicity, lipophilicity, and capacity to form tetramers) in combination with reactive conditions (melittin concentration, salt concentration, pH, and temperature) are responsible for the targeted destruction of bacterial cells and apparent tolerance towards own tissue cells. Considering that melittin is an important current and, importantly, potential broad-spectrum medication, a thorough understanding of the observed phenomena may significantly increase its use in clinical practice.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/GA24-10662S" target="_blank" >GA24-10662S: Defence reactions against Habrobracon hebetor wasp and honeybee venoms: mechanism of their action</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2024

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Comparative Biochemistry and Physiology Part C

  • ISSN

    1532-0456

  • e-ISSN

    1878-1659

  • Volume of the periodical

    281

  • Issue of the periodical within the volume

    JUL 01

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    109928

  • UT code for WoS article

    001218562100002

  • EID of the result in the Scopus database

    2-s2.0-85190996531