Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00619033" target="_blank" >RIV/60077344:_____/25:00619033 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/25:00619033 RIV/60076658:12310/25:43909896 RIV/61988987:17310/25:A2603CM2 RIV/00216208:11310/25:10503687
Result on the web
<a href="https://www.nature.com/articles/s41594-024-01450-z" target="_blank" >https://www.nature.com/articles/s41594-024-01450-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41594-024-01450-z" target="_blank" >10.1038/s41594-024-01450-z</a>
Alternative languages
Result language
angličtina
Original language name
Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough
Original language description
Transfer RNAs (tRNAs) serve as a dictionary for the ribosome translating the genetic message from mRNA into a polypeptide chain. In addition to this canonical role, tRNAs are involved in other processes such as programmed stop codon readthrough (SC-RT). There, tRNAs with near-cognate anticodons to stop codons must outcompete release factors and incorporate into the ribosomal decoding center to prevent termination and allow translation to continue. However, not all near-cognate tRNAs promote efficient SC-RT. Here, with the help of Saccharomyces cerevisiae and Trypanosomabrucei, we demonstrate that those tRNAs that promote efficient SC-RT establish critical contacts between their anticodon stem (AS) and ribosomal proteins Rps30/eS30 and Rps25/eS25 forming the decoding site. Unexpectedly, the length and well-defined nature of the AS determine the strength of these contacts, which is reflected in organisms with reassigned stop codons. These findings open an unexplored direction in tRNA biology that should facilitate the design of artificial tRNAs with specifically altered decoding abilities.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Structural & Molecular Biology
ISSN
1545-9993
e-ISSN
1545-9985
Volume of the periodical
32
Issue of the periodical within the volume
January 13
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
662-674
UT code for WoS article
001395439100001
EID of the result in the Scopus database
2-s2.0-85217547932