Detection of tick-borne encephalitis virus RNA in patient samples at different stages of infection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00619444" target="_blank" >RIV/60077344:_____/25:00619444 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/25:43909975 RIV/00216224:14310/25:00144737 RIV/00027162:_____/25:N0000028

Result on the web

<a href="https://doi.org/10.1016/j.jinf.2025.106481" target="_blank" >https://doi.org/10.1016/j.jinf.2025.106481</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jinf.2025.106481" target="_blank" >10.1016/j.jinf.2025.106481</a>

Result language

angličtina

Original language name

Detection of tick-borne encephalitis virus RNA in patient samples at different stages of infection

Original language description

Objectives: The aim of the study was to evaluate the efficiency of molecular diagnostics of tick-borne encephalitis (TBE) and to correlate viral RNA (vRNA) detection with the clinical and laboratory data. Methods: Clinical samples from 1125 patients from South Bohemia, Czech Republic, a highly endemic TBE region, were screened for TBE virus (TBEV) RNA by RT-qPCR. Samples included blood, serum, cerebrospinal fluid (CSF), and urine. Results: TBEV RNA was detected in 14 patients with clinically proven TBE. TBEV RNA was most frequently detected in sera during early infection (11/37 patients tested, 29.7%) but decreased with rising IgG antibody response (3/228, 1.3%). Detection in CSF and urine was infrequent (1/30, 3.3% and 1/52, 1.9%, respectively). Additionally, five patients initially not diagnosed with TBE were retrospectively found to have TBEV RNA in serum, indicating possible underdiagnosis, particularly in mild or atypical presentations. The study also highlighted the diagnostic challenge of an immunocompromised patient whose delayed antibody response hindered timely diagnosis. In such cases, RT-qPCR could significantly shorten the diagnostic timeline. Conclusions: These findings underscore the value of early RNA detection in improving the diagnosis of TBE and may in the future facilitate the early administration of potential treatment, thereby improving patient outcomes. (c) 2025 The Author(s). Published by Elsevier Ltd on behalf of The British Infection Association. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Infection

ISSN

0163-4453

e-ISSN

1532-2742

Volume of the periodical

90

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

106481

UT code for WoS article

001468963600001

EID of the result in the Scopus database

2-s2.0-105002224578