Mosquito- and tick-borne orthoflaviviruses cross an in vitro endothelial-astrocyte barrier

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00637543" target="_blank" >RIV/60077344:_____/25:00637543 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/25:00141852 RIV/00027162:_____/25:N0000057

Result on the web

<a href="http://10.3389/fcimb.2025.1624636" target="_blank" >http://10.3389/fcimb.2025.1624636</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcimb.2025.1624636" target="_blank" >10.3389/fcimb.2025.1624636</a>

Result language

angličtina

Original language name

Mosquito- and tick-borne orthoflaviviruses cross an in vitro endothelial-astrocyte barrier

Original language description

Introduction The genus Orthoflavivirus of the Flaviviridae family includes several notable pathogens such as mosquito-borne West-Nile virus (Orthoflavivirus nilense, WNV) and Tick-borne encephalitis virus (Orthoflavivirus encephalitidis, TBEV) that are highly neurotropic and may cause severe neurological disease leading to lifelong disabilities, coma and death. These viruses have developed mechanisms to breach the compact blood-brain barrier (BBB) and establish infection within the central nervous system (CNS). Nevertheless, neuroinvasive mechanisms of orthoflaviviruses remain poorly understood. Complex anatomy of the CNS and the organization of the BBB is a major challenge to study neuroinvasion of orthoflaviviruses in vivo. Therefore, in vitro BBB models are useful tools to study direct interaction of viruses with the endothelial barrier.Methods In this study, we employed an in vitro transwell BBB model comprising primary mouse brain microvascular endothelial cells and astrocytes to compare the ability of mosquito-borne and tick-borne orthoflaviviruses to cross a compact endothelial barrier and reach the basolateral compartment of the transwell system. The influence of virus inoculation on the barrier properties was determined by measuring transendothelial electrical resistance (TEER).Results The results demonstrate that while pathogenic WNV and TBEV cross the endothelial barrier the ability of low pathogenic Usutu virus (USUV) and Langat virus (LGTV) was inconsistent. All viruses tested display virus replication within the endothelial cells. Nevertheless, virus replication did not affect the barrier function of endothelial cells as demonstrated by sustained TEER and absence of leakage of high molecular weight dextran molecules through the endothelial barrier even at several hours post infection.Discussion Our findings indicate that orthoflaviviruses can infect the endothelial cells, replicate within them without affecting the cells and its barrier function. Nevertheless, only pathogenic WNV and TBEV showed the ability to cross the endothelial barrier and reach the basolateral compartment.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/GA23-08039S" target="_blank" >GA23-08039S: Neuropathogenesis linked to NS1 protein of tick-borne encephalitis virus</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Cellular and Infection Microbiology

ISSN

2235-2988

e-ISSN

2235-2988

Volume of the periodical

15

Issue of the periodical within the volume

JUL 2025

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

1624636

UT code for WoS article

001529286000001

EID of the result in the Scopus database

2-s2.0-105010965183