Eukaryote-Wide Distribution of a Family of Longin Domain-Containing GAP Complexes for Small GTPases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00637874" target="_blank" >RIV/60077344:_____/25:00637874 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1111/tra.70016" target="_blank" >https://doi.org/10.1111/tra.70016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tra.70016" target="_blank" >10.1111/tra.70016</a>
Alternative languages
Result language
angličtina
Original language name
Eukaryote-Wide Distribution of a Family of Longin Domain-Containing GAP Complexes for Small GTPases
Original language description
Arf and Rab family small GTPases and their regulators, GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs), play a central role in membrane trafficking. In this study, we focused on a recently reported GAP for Arf (and potentially Rab) proteins, the CSW complex, a part of a small family of longin domain-containing proteins that form complexes with GAP activity. This family also includes folliculin and GATOR1, which are GAPs for the Rag/Gtr GTPases. All three complexes are associated with lysosomes and play a role in nutrient signaling, the latter two being directly involved in the mTOR pathway. The role of CSW is not clear, but in addition to having GAP activity on Arf proteins in vitro, its mutation causes severe neurodegenerative diseases. Here we update the reported pan-eukaryotic presence of folliculin and GATOR1, and demonstrate that CSW is also found throughout eukaryotes, though with sporadic distribution. We identify highly conserved motifs in all CSW subunits, some shared with the catalytic subunits of folliculin and GATOR1, that provide new potential avenues for experimental exploration. Remarkably, one such conserved sequence, the GP motif, is also found in structurally related longin proteins present in the archaeal ancestor of eukaryotes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Traffic
ISSN
1398-9219
e-ISSN
1600-0854
Volume of the periodical
26
Issue of the periodical within the volume
7-9
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
e70016
UT code for WoS article
001536285600001
EID of the result in the Scopus database
2-s2.0-105011851823