Biogenesis of Inner Mitochondrial Membrane Proteins in Trypanosoma brucei

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00642777" target="_blank" >RIV/60077344:_____/25:00642777 - isvavai.cz</a>
Result on the web
<a href="https://drive.google.com/file/d/1VBqtyqlV1Jc9A3c89jJNFkVdGGSv985u/view" target="_blank" >https://drive.google.com/file/d/1VBqtyqlV1Jc9A3c89jJNFkVdGGSv985u/view</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Biogenesis of Inner Mitochondrial Membrane Proteins in Trypanosoma brucei
Original language description
The mitochondrial insertase Oxa1 is essential for the insertion of nuclear- and mitochondrial-encoded transmembrane proteins and the biogenesis of respiratory chain (RC) complexes at the inner mitochondrial membrane (IMM). Trypanosoma brucei, a parasitic protist with a single mitochondrion, encodes three putative TbOxa1 paralogs. Whether these paralogs have substrate specificity or specialized roles in IMM biogenesis remains unclear. Using CRISPR/Cas9-mediated ablation in procyclic form (PF) T. brucei, we observed reduced cell viability, accumulation of RC complex subunit precursors, and impaired RC complex integrity upon loss of individual TbOxa1s. Submitochondrial fractionation combined with label-free quantitative mass spectrometry revealed distinct proteomic alterations across mitochondrial subcompartments, indicating both shared and specific roles of TbOxa1 paralogs in IMM protein biogenesis, particularly affecting RC complex components.
Czech name
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Czech description
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Project
<a href="/en/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA for therapy</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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