Molecular cloning, structure and bait region splice variants of alpha-2-macroglobulin from the soft tick Ornithodoros moubata.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077409%3A_____%2F03%3A59033034" target="_blank" >RIV/60077409:_____/03:59033034 - isvavai.cz</a>
Alternative codes found
RIV/60077409:_____/03:00157647
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Molecular cloning, structure and bait region splice variants of alpha-2-macroglobulin from the soft tick Ornithodoros moubata.
Original language description
The sequence of alpha-2-macroglobulin (alpha-2M) from the soft tick Ornithodoros moubata (TAM) was determined. The TAM cDNA sequence is 4944 bp long and contains one open reading frame coding for a protein precursor composed of 1494 amino-acid residues,including a 24-residue signal sequence. The mature protein is cleaved into two subunits. Phylogeny analysis revealed that TAM is closely related to Limulus alpha-2M and displays the highest similarity to the partial sequence of alpha-2M from hard tick Ixodes scapularis. Four variants of the TAM bait region differing only in a short central segment were found; our data indicate that TAM exists as a single-copy gene in the tick genome and its bait region variants likely arise by alternative splicing. TAMis produced by tick hemocytes and it is also significantly expressed in salivary glands. TAM mRNA levels were shown to be up-regulated upon blood meal.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Insect Biochemistry and Molecular Biology
ISSN
0965-1748
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
841-851
UT code for WoS article
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EID of the result in the Scopus database
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