Ethanol induced apoptosis in human HL-60 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG16__%2F00%3A00000376" target="_blank" >RIV/60162694:G16__/00:00000376 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/00:00000673

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Ethanol induced apoptosis in human HL-60 cells

Original language description

In this study flow cytometric and morphologic methods of apoptosis detection in human promyelocytic leukemia cell line HL-60 were compared. HL-60 cells were harvested at 4, 7, 16, 24 a 48 hours after induction of apoptosis by 3 % ethanol. Little changeswere observed both by flow cytometry (decrease of forward scatter, increase of unprocessed cells staining with APO 2.7 antibody) and viability determination by Trypan-blue staining until after 7 hours. However, after 4 hours morphologic changes were observed in the nuclear and cytoplasmic structures using Diff-Quik stained cytospin preparations and standard light microscopic techniques (50% apoptotic cells). The same results were obtained by flow cytometric measurement of sub-diploid DNA content (sub-G1 cells), and an increase of staining with APO 2.7 antibody in cells permeabilised by digitonin prior to staining. After 7 hours almost all cells exhibited apoptotic morphology. After 16 hours the cell size (forward scatter) decreased sig

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/MO66020398129" target="_blank" >MO66020398129: Radiosenzitivity of hematopoietic progenitors (CD34+/AC133+) and effect of citokines on expansion ex vivo.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

General Physiology and Biophysics

ISSN

0231-5882

e-ISSN

—

Volume of the periodical

19

Issue of the periodical within the volume

—

Country of publishing house

XX - stateless person

Number of pages

14

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—